Composite

Part:BBa_K4144044:Design

Designed by: Pi Ruiqi   Group: iGEM22_WHU-China   (2022-10-11)


Lac double selector with fluorescence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since using only fluorescence to evaluate the state of the promoter is inaccurate and time-consuming, we decided to integrate the selection into conditional culture. By adding sacB and KanR as reporter gene, we are able to deliver the selection along with selective sulture.


Source

Lac promoter refers to R0010; mRFP1 refers to BBa_E1010; sacB refers to BBa_K322921; KanR and its RBS refers to BBa_K4144041; double terminators refer to BBa_B0015; All sessions come from previous parts but with secondary combination.

References